databot
IOS/Android Smart Device
Plastic bag – 1 quart size,
ziplock preferred!
Drinking straw
Investigations
Life Science
databot Investigations | Life Science | Breathe: Exploring the Human Respiratory System
Copyright (c) 2026 databot LLC
CO2
Breathe: Exploring the Human
Respiratory System
Oxygen is necessary for cells to release energy from
food through a process called cellular respiration.
During this process, carbon dioxide is produced as
a waste gas.
Air travels through:
Nose or mouth
Trachea
Bronchial tubes
Bronchioles
Alveoli
In the alveoli, oxygen moves into the bloodstream
and carbon dioxide moves out.
When you exercise:
Muscles need more energy
Cells use more oxygen
Cells produce more carbon dioxide
Your body responds by increasing breathing rate and
depth. This automatic adjustment helps maintain
homeostasis — a stable internal balance of gases in
the body.
Overview
Background
What You Will Need/Prep
Test your databot™ connection.
You will be prompted to select
and connect to databot™ each
time you launch an experiment.
If there are two or more
databot™'s listed, the one
closest to your device will be
highlighted.
You breathe about 20,000 times every day —
without even thinking about it. But what actually
changes when you exercise? What happens inside
your body when you hold your breath? Today, you
won’t just talk about breathing — you will see real
data from your own body using databot.
Install Vizeey™ on your
Smart device.
Study the background
information and terms and
prepare to explore!
Grades: Middle School
Time: 45 Minutes
Subject: Life Science
Topics: Respiratory System, O2, CO2,
Homeostasis, Effects of exercise on
breathing
Scan the QR code to load
the experiment.
Investigations
Life Science
databot Investigations | Life Science | Breathe: Exploring the Human Respiratory System
CO2
Important Terms
Learning Objectives
In this investigation you will master the following knowledge and skills:
Visualize, collect and analyze data
Measure and record CO₂ levels in ppm.
Understand that the human respiratory system is one system of many in the human
body, and it is responsible for breathing!
Compare CO₂ levels at rest and after exercise
Explain why CO₂ levels change
Connect breathing changes to cellular energy needs
Alveoli: Tiny air sacs in your lungs that facilitate the exchange of oxygen into your
bloodstream and carbon dioxide out of it.
Bronchial Tubes: Branch off your trachea and carry the air you inhale into your lungs.
Bronchiole: Smaller passages off your bronchial tubes that lead to the tiny air sacs known
as alveoli where gas exchange takes place.
Carbon Dioxide (CO₂): A colorless, odorless gas naturally present in the air you breathe.
A waste gas produced during cellular respiration, which is absorbed by plants in
photosynthesis.
Homeostasis: Your body’s systems and processes that help maintain a balance of things
like your oxygen and CO2 levels.
Parts per million - (ppm): Usually describes the concentration of something in air, water or
soil. The term ppm expresses the number of units (part) of a given substance that exists as
a portion of a greater substance comprised of one million parts.
Respiration: Facilitates the production of energy in the body through the process of
breathing in oxygen and expelling carbon dioxide.
Respiratory System: your lungs, airway, and associated muscles, are responsible for
breathing – taking in oxygen and expelling carbon dioxide.
Trachea, or windpipe: A large tube that conveys air to and from your lungs. It is an
important part of your respiratory system.
In this lab, you will measure the carbon dioxide (CO₂) in your
breath using databot™. You will discover how your body responds
to exercise and how your respiratory system works to keep you
alive and balanced.
Using Vizeey
In order to work with the experiment you need to launch Vizeey application and click on + in
the upper right corner.
Then select “Add experiment from QR code” and scan the QR code prepared for this
experiment. Your experiment will appear in the list.
Investigations
Life Science
databot Investigations | Life Science | Breathe: Exploring the Human Respiratory System
CO2
Alveoli
Bronchial tubes (Bronchi)
Bronchioles
Lungs (Right and Left)
Trachea
Alveoli
When you start the experiment you will be immediately offered to connect to your databot.
Make sure that databot is enabled.
Look for these important terms that identify important
parts of your respiratory system on the diagram to the
right. Trace their location on your own body. You are
about to give your respiratory system a workout –
good luck!
Part 1: Initial Observations and Questions
Why do you breathe faster when you run? Write your thoughts below.
Investigations
Life Science
databot Investigations | Life Science | Breathe: Exploring the Human Respiratory System
Do you think you produce more CO₂ when resting or exercising?
CO2
Predict how exercise will affect CO₂ levels.
Write your prediction below.
Part 2: Hypothesis
Part 3: Experiment Procedure
Before starting the experiment:
Prepare databot, plastic bag, and a straw.
Make sure your databot is fully charged.
What do you think will happen to CO₂ levels after holding your breath?
Prepare to hop around a bit so you can test your CO2 level before and after exercise!
Turn on your charged databot™
(using the small button on the left side) and place it in your ziplock bag.
Zip the bag closed except for a small space for a straw.
Open the Vizeey™ app on your smart device and click on the Breathe experiment
To start and to pause the experiment use:
Investigations
Life Science
databot Investigations | Life Science | Breathe: Exploring the Human Respiratory System
CO2
Activity 1: CO₂ at Rest vs. Exercise
Measure CO₂ at Rest
Start your experiment with button
Exhale gently into the bag through the straw, the bag will inflate and air will leak out
around the straw which is fine.
Write down the highest level displayed by databot™ in the “Before” space into the
table below.
Exercise
Now exercise for two full minutes doing jumping jacks, running in place, hopping,
etc. to get your heart rate up. Work as hard as you can and think about your
breathing. Stop after two minutes.
Clear your data using and start fresh measurements. Now breathe again into
the bag with databot.
Write down the new value and calculate any change.
Repeat for 3 trials.
Part 3: Experiment Procedure
Activity 2: Holding Your Breath
Measure CO₂ at Rest and write down the highest level displayed in the “Before” space.
Hold breath for 30 seconds (or comfortable time).
Safety:
Stop immediately if dizzy.
Do not compete for longest breath.
Sit down during breath-holding test.
Clear your data and start fresh measurements.
Trial | CO₂ Before | CO₂ After | Net Change |
1 | |||
2 | |||
3 | |||
Average |
Data Collection Table: CO₂ at Rest vs. Exercise
Part 3: Experiment Procedure
Part 4: Data Analysis
Now that you have collected your data, it is time to analyze your results.
Create a bar graph to visually compare your results:
X-axis: Condition - Resting CO₂, Post-exercise CO₂, Post-breath-holding CO₂;
Y-axis: Maximum CO₂ (ppm)
Investigations
Life Science
databot Investigations | Life Science | Breathe: Exploring the Human Respiratory System
Trial | CO₂ Before | CO₂ After | Net Change |
1 | |||
2 | |||
3 | |||
Average |
Data Collection Table: CO₂ and Breath Holding
CO2
Breathe again into the bag with databot. Write down the new value and calculate any
change.
Repeat the experiment twice more and record the values.
Part 5: Concept Questions
How does this lab demonstrate that the body is a system of interacting parts?
Why does your breathing rate change automatically?
Why is carbon dioxide considered a waste product?
Investigations
Life Science
databot Investigations | Life Science | Breathe: Exploring the Human Respiratory System
CO2
Part 4: Data Analysis
Did CO₂ increase after exercise?
Which caused a greater change: exercise or breath-holding?
Why does exercise increase CO₂ production?
How does your body maintain homeostasis?
Part 6: Reflection
Did the data match your prediction?
What surprised you about your results?